Transcriptional Regulatory Networks
Abstract: In the budding yeast Saccharomyces cerevisiae, large-scale gene deletion analysis has shown that over 80% of the ~6200 yeast genes are non-essential, implying that many genes and pathways in eukaryotic cells must be functionally redundant or buffered from phenotypic consequences following genetic perturbation. I am interested in exploring this functional redundancy, especially as it relates to transcriptional regulatory networks. A number of uncharacterized open reading frames (ORFs) have sequence homology to well known families of transcription factors. Using high-throughput genetic analysis (Synthetic Genetic Array Analysis, or SGA), DNA microarrays, and the recently developed ProtoArray (an array based method for high-throughput identification of protein-protein interactions), I would like to connect one or more of these uncharacterized putative regulatory genes with a new or existing physiologically significant pathway.
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